1. Name and Synonyms

The product is officially named Colistin Sulphate, and it may also be known as Colistin Methanesulfonate in certain contexts. These synonyms are commonly used in different research and clinical settings, aiding in the clear identification of the product across veterinary applications.

2. Chemical Identification

Colistin Sulphate is identified with the CAS number 1264-72-8, providing a unique identifier in the pharmaceutical and veterinary industries. This CAS number helps ensure precision in research, production, and quality control.

3. Appearance and Physical Properties

Colistin Sulphate appears as a white or almost white, hygroscopic powder. This physical property aids in product identification and quality inspection throughout manufacturing and distribution.

4. Purity and Solubility

The product has a high purity level of ≥ 98%, ensuring optimal effectiveness in veterinary use. Colistin Sulphate is freely soluble in water, slightly soluble in ethanol, and almost insoluble in solvents like acetone, chloroform, and ether. This solubility profile supports its targeted use in water-soluble formulations for livestock applications.

5. Molecular Structure

The molecular formula of Colistin Sulphate is C₅₂H₁₀₀N₁₆O₁₃S, with a molecular weight of approximately 1155 g/mol. This structure is key to its antibacterial activity, making Colistin Sulphate highly effective against specific Gram-negative bacteria in veterinary medicine.

1. Antibiotic Type

Colistin Sulphate belongs to the polymyxin class of antibiotics. Polymyxins are a specialized group of antibiotics with potent activity primarily against Gram-negative bacteria. They exert their antibacterial effect by disrupting the bacterial cell membrane, making them effective against certain multidrug-resistant bacteria.

2. Antibacterial Spectrum

2.1 Gram-Negative Bacteria

Colistin Sulphate has a strong inhibitory effect on a variety of Gram-negative bacteria, including Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella, and Enterobacter species. Escherichia coli, a common intestinal flora, can cause infections under certain circumstances, while Pseudomonas aeruginosa is known for causing serious infections in animals with compromised immunity. Klebsiella pneumoniae can lead to respiratory infections, and Salmonella is a primary cause of food poisoning and intestinal diseases. Colistin Sulphate’s potency against these pathogens makes it valuable in treating intestinal infections, septicemia, and respiratory diseases in veterinary applications.

2.2 Limited Activity Against Gram-Positive Bacteria

Colistin Sulphate is primarily active against Gram-negative bacteria and has limited or no efficacy against Gram-positive bacteria such as staphylococci and streptococci. This specificity makes it particularly useful in situations where targeted action against Gram-negative pathogens is needed without affecting the normal Gram-positive flora.

2.3 Other Microorganisms

Colistin Sulphate has little to no activity against mycoplasmas, rickettsiae, or spirochetes, as it primarily targets the outer membrane structure unique to Gram-negative bacteria. Therefore, it is not typically used in infections caused by these microorganisms.

3. Comparison of Antibacterial Activity

The antibacterial activity of Colistin Sulphate is particularly notable against multidrug-resistant Gram-negative bacteria, such as those producing extended-spectrum beta-lactamases (ESBLs) or carbapenemases. Its potent cell membrane-disrupting mechanism makes it a valuable choice in situations where other antibiotics may not be effective. Compared to other antibiotics, Colistin Sulphate has a highly selective and powerful effect on Gram-negative bacteria.

4. Application Fields

Colistin Sulphate is commonly used in veterinary practice, especially in treating infections in livestock and poultry. In animal husbandry, it is widely applied to treat intestinal infections and septicemia in pigs, poultry, and cattle. Due to its effectiveness against Gram-negative enteric bacteria, it is especially useful in managing outbreaks of colibacillosis and salmonellosis in herds and flocks. Colistin Sulphate’s ability to specifically target harmful Gram-negative bacteria without affecting beneficial Gram-positive organisms makes it an essential part of disease management in intensive breeding environments.

1. Shelf Life

Colistin Sulphate has a shelf life of 2 years when stored under recommended conditions. Within this period, the product maintains its quality, effectiveness, and stability, ensuring it provides the intended antibacterial effects in veterinary applications.

2. Testing Indicators and Standards

2.1 Appearance

Colistin Sulphate should appear as a white or almost white, hygroscopic powder. Any deviation from this appearance may indicate potential quality issues, and this serves as a basic quality control measure.

2.2 Identification

The product is identified through multiple analytical methods, including High-Performance Liquid Chromatography (HPLC), which uses retention time (tR) to confirm the identity of Colistin Sulphate. Additionally, chemical tests such as sulfate and chloride reactions verify its purity and authenticity from different perspectives.

2.3 pH Value

The pH of a 1% solution of Colistin Sulphate in water should range between 5.5 and 7.5. Maintaining this pH range is essential for ensuring stability and biological activity, as deviations may impact the product’s effectiveness.

2.4 Specific Absorbance

At 276 nm, the specific absorbance of Colistin Sulphate should meet the required range for quality assurance. This measurement verifies product purity and confirms that it meets the expected absorbance profile.

2.5 Light Absorbing Impurities

At 420 nm, light-absorbing impurities should not exceed specified limits. Controlling these impurities helps maintain product stability and minimizes any impact on effectiveness.

2.6 Related Substances Content

Strict limits are placed on related substances to ensure high purity. For instance, the presence of related polymyxins and other impurities must be below 2.0% to maintain the product’s quality and effectiveness in veterinary applications.

2.7 Heavy Metals Content

The heavy metals content in Colistin Sulphate should not exceed 20 ppm. Excessive heavy metals can be harmful to animal health, so maintaining this low level is essential for product safety.

2.8 Water Content

Water content is controlled to be between 5.0% and 8.0%. Excess moisture can affect the stability and quality of Colistin Sulphate, so maintaining it within this range ensures product consistency and prevents caking or degradation.

2.9 Sulfated Ash Content

The sulfated ash content should not exceed 0.1%. This parameter measures inorganic residue, and a low ash content indicates high product purity, free from unwanted contaminants.

2.10 Content Determination

The potency of Colistin Sulphate is determined based on the active content of Colistin base, with the required range between 98.0% and 102.0%. Ensuring the active content meets this specification guarantees that the product delivers effective results in veterinary use.

1. Packaging

The product is packaged in fiber drums, with two specifications of 15kg/drum and 25kg/drum. This packaging method can effectively protect the product from external environmental influences such as moisture and oxidation, and is also convenient for product storage and transportation.

2. Transportation Modes

2.1 Express Transportation

It can be transported by DHL, TNT, Fedex, etc., international well-known express services. These express services are fast and reliable and can deliver the product to customers in a relatively short time.

2.2 Air and Sea Transportation

For large quantities of goods, air or sea transportation can be chosen. Air transportation is fast but relatively expensive; sea transportation is less expensive but takes a longer time. According to the customer’s needs and actual situation, a suitable transportation mode can be flexibly chosen.

3. Delivery Time

After receiving payment, the product will be delivered within 2 – 3 days. This fast delivery time can meet the urgent needs of customers and improve customer satisfaction.

Q1: How to order this product?

A: You can chat with online service directly.

You can also contact us in the following ways.

Wechat:+86-18663729963, +86-13589106478

Skype: sunnylee886

Email:maggie@hope-biotech.com

sunny@hope-biotech.com

Mobile:+86-18663729963, +86-13589106478

Q2: What’s the MOQ?

A: The MOQ is 0.5kg, you can also tell us the quantity you want.

Q3: How about your company’s certification?

A: GMP, ISO, FSC, CEP etc.